培養Mycoplasma pneumoniae 最適當的環境為:
pH 4.0~5.0,含\mathrm{CO}_{2},2 days
\mathrm{CO}_{2}
pH 6.5~7.5,含\mathrm{CO}_{2},14 days
pH 6.5~7.5,Anaerobic,2 days
pH 4.0~5.0,Anaerobic,14 days